Fig. 2. Two-hybrid interaction between XMog1 and Ran. The growth of yeast on plates
with (His+) or without (His-) histidine is shown. Yeast
were co-transformed with a plasmid encoding the activation domain of Gal4
fused to XMog1 (pAD-XMog1) and a second plasmid encoding the DNA-binding
domain of GAL4 (pDB) fused to wild-type Ran, RanQ69L, RanT24N or p53 as a
negative control. Co-transformation with Gal4AD/SV40 large T antigen and
Gal4BD/p53 was used as a positive control. The diagram illustrates the
position of each co-transformant on the plates.
