Fig. 3. KNOLLE protein immunolocalisation in 35S::KN seedling roots.
Seedlings were grown on 1% agar with 0.5x MS salts and 1% sucose.
Dark-field images of wild-type root tip (B) and root differentiation zone (E)
are shown as reference for the epifluorescence images of roots homozygous for
the 35S::KN transgene (A,D,G) and of wild-type control roots (C,F,H).
Broken lines delineate root tips (A,C) and root hairs and lateral surface of
root (D,F). (A,C) Root tip with KN-labelled cell plates (arrowheads). KN is
also expressed in the non-proliferating cells of the central cylinder in the
transgenic root (A; see also D) but not in the wild-type root (C). (D,G,F,H)
Differentiation zone of the root. KN is expressed in the central cylinder (cr)
and in root hair tips (arrowheads) of transgenic roots (D,G) but not in
wild-type control roots (F,H). (D,F) Brightness of images increased; (F)
maximum brightness to visualise the wild-type root in the absence of the KN
signal. (G,H) Original images; (H) wild-type root is not visible. (I-L) GUS
staining (2 hours, blue colour) to visualise 35S promoter activity in
6-day-old 35S::GUS transgenic seedlings. (I) Seedling with high GUS
activity in root tip, root hairs and tips of the cotyledons. Boxed areas are
magnified in J (young root hair), K (central part of the root) and L (root
tip). Scale bar: 50 µm.
