Fig. 1. Constructs for KNOLLE expression in transgenic plants. The constructs were
cloned into the multiple cloning site of the pBar transformation vectors
indicated (see Materials and Methods). Numbers indicate distances (in bp) from
ATG (+1) of the KNOLLE gene. The arrow above the gene indicates the
orientation of transcription. KNRescue (top), genomic DNA fragment containing
the KNOLLE gene and fragments of the adjacent genes. Thiored., gene
encoding thioredoxin-1-like protein (AF144387); VI, hypothetical gene possibly
encoding a protein similar to violaxanthin de-epoxidase (CAB59211.1); AP3::KN1
(middle), AP3 promoter (pAP3) fused to a genomic DNA fragment with
KNOLLE intron; p35S::KN (bottom), CaMV 35S promoter (p35S) of the
pBAR-35S vector fused to a genomic DNA fragment without KNOLLE
intron. Restriction sites: E, EcoRI; P, PstI; Sc,
SacI; Sn, SnaI; X, XbaI. polyA, poly-adenylation
site; L, left border of the T-DNA; R, right border of the T-DNA.
