Fig. 8. Reduction of PYK2 focal adhesion targeting by FAK. (a) First,
fak-/- fibroblasts were transiently transfected with the
empty vector (A-C) or vectors encoding FAK (D-F) or FRNK (G-I). They were then
stained with polyclonal antibodies against PYK2 (UBI) (A,D,G) or monoclonal
antibodies against paxillin (B) or FAK (E,H); C, F and I are overlay pictures.
Arrows indicate the FAK- or FRNK-expressing cells. Bar, 50
µm. (b) Summary of results from (a). More than 100 transfected cells were
examined for endogenous PYK2 subcellular localization. Although most
fak-/- cells (>90%) exhibited PYK2 focal adhesion
targeting (+++), most fak-/- cells expressing FAK
or FRNK (>80%) showed reduced staining intensity (+) compared with
fak-/- cells without FAK or FRNK
expression in the same image field.
