Fig. 6. mRNA levels are decreased in the wat1 mutant. (A) Northern
analysis of tbp1+ transcripts with specific probes for
intron and exon sequences. Wild-type (lanes 1 to 3), ts prp2 mutants
(lanes 4 to 6) or wat1-deleted cells (lanes 7 to 9) were shifted from
26°C to 36°C, and total RNAs were prepared at 0 (lanes 1, 4 and 7), 2
hours (lanes 2, 5 and 8) and 4 hours (lanes 3, 6 and 9). 20 µg RNA was run
in each lane. Northern hybridisation was performed using probes specific for
intron (upper) or exon sequence (lower) corresponding to the
tbp1+ gene. (B) Steady state transcript levels of various
genes. RNA samples from wild-type (lanes 1 and 2) or wat1-deleted
cells (lanes 3 to 5) prepared in A were used to examine transcript levels of
nda2+, cdc2+,
cig2+ and act1+. Equal loading (20
µg) of RNA was confirmed with ethidium bromide staining of the gel (not
shown).
