Fig. 4. Immunoblots of non-denaturing gels of lysates from corneal epithelial cells transfected with those constructs that localize to the nucleus (HM, D14, D13 and D11) and to the cytoplasm (D10 as an example), reacted with anti-myc antibody 9E10. Construct HM produced a protein band migrating similarly to the endogenous ferritin at 
260 kDa. The high molecular weight bands were also observed in cells transfected with D14 and D13. Some low molecular weight bands migrating at 22 kDa were seen in D14- and D13-transfected cells, showing the monomeric myc-tagged ferritin. Only the low molecular weight bands were seen in D11- and D10-transfected cells. The faint high molecular weight band seen in D11 (arrow) was from the nonspecific binding of the secondary antibody (see text).
