Fig. 3. As the concentration of anti-EGFR blocking antibody increases, the concentration of EGF in the bulk medium increases. Cells induced to secrete ligand at a rate of approximately 600 moleculesxcell^-1xminute^-1using tetracycline were allowed to condition their medium in the presence of 0.0, 0.05, 0.1, 0.5 or 5.0 µg/ml 225 mAb for 12 hours. The medium was collected and assayed for EGF by ELISA. The error bars represent the s.d. of one experiment in duplicate.

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