QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online 27 February 2007
doi: 10.1242/jcs.03414

This Article Summary Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Abdu, U. Articles by Schüpbach, T. Search for Related Content PubMed PubMed Citation Articles by Abdu, U. Articles by Schüpbach, T.

An essential role for Drosophila hus1 in somatic and meiotic DNA damage responses

Uri Abdu^1,*,, Martha Klovstad^2,*, Veronika Butin-Israeli¹, Anna Bakhrat¹ and Trudi Schüpbach²

¹ Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben-Gurion University, Beer-Sheva, 84105, Israel
² Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA

View larger version (46K): [in this window] [in a new window]   Fig. 1. Detection of the interaction between Hus1 and Rad9 or Rad1. 1, L40 bearing Hus1 in pHybLex/Zeo vector and Rad9 in pYESTrp2 vector; 2, L40 bearing Hus1 in pHybLex/Zeo vector and Rad1 in pYESTrp2 vector; 3, L40 bearing Rad9 in pYESTrp2 vector and an empty pHybLex/Zeo vector; 4, L40 bearing Hus1 in pHybLex/Zeo vector and an empty pYESTrp2 vector; 5, L40 bearing Rad1 in pYESTrp2 vector and an empty pHybLex/Zeo vector. (A) Non-selective medium for detection of interaction; (B) The activation of the HIS promoter was tested on plates without histidine. (C) Activation of the lacZ promoter by assay of -galactosidase activity. Hus1 interacted either with Rad9 (B1,C1) or Rad1 (B2,C2).

View larger version (11K): [in this window] [in a new window]   Fig. 2. RT-PCR detection of hus1, rad9 and rad1 transcripts in wild-type and in hus137 mutant ovaries. (A) Identification of transcripts in wild-type ovaries. Lane 1, hus1; lane 2, rad1; lane 3, rad9. (B) Detection of rad9 and hus1, transcripts in wild-type (lane 1) and in hus137 (lane 2) mutant ovaries.

View larger version (18K): [in this window] [in a new window]   Fig. 3. hus1 mutant larvae accumulate aneuploid nuclei after MMS treatment. (A,B) Chromosome spreads of (A) wild-type neuroblast and (B) hus137 mutant nucleus lacking one sex chromosome. (C) Frequencies of aneuploid nuclei after MMS treatment. Bars indicate standard deviations between the average percentage aneuploid nuclei from four brains from two separate experiments.

View larger version (42K): [in this window] [in a new window]   Fig. 4. hus1 is not required for the G2-M checkpoint in the developing wing disc. (A-F) Larvae were mock-irradiated or irradiated with 4000 rad and allowed to recover for 1 hour before detection prior to fixation for phosopho-histone H3 staining. (G) Number of mitotic cells in imaginal wing discs. Bars indicate standard deviations in the average number of mitotic cells from at least five wing discs.

View larger version (84K): [in this window] [in a new window]   Fig. 5. hus1 is not required for post-irradiation induction of apoptosis in the developing wing disc. (A-F) Larva were mock-irradiated or irradiated with 4000 rad and allowed to recover for 4 hours before detection of apoptosis with Acridine Orange. Representative discs are shown; at least fifteen discs were examined for each condition.

View larger version (132K): [in this window] [in a new window]   Fig. 6. Organization of the DNA in the oocyte nucleus in wild-type and hus1 mutants. Egg chambers with DNA shown in green and nuclear membranes in red. Insets show a higher magnification of the oocyte DNA. (A) Wild type; (B) hus37; (C) hus37/Df(3R)110; (D) spn-BBU. The wild-type karysome is a sphere near the center of the nucleus, whereas the mutant karysomes are crescent-shaped DNA masses near the nuclear periphery.