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First published online 23 March 2004
doi: 10.1242/jcs.01054

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Length control is determined by the pattern of cytoskeleton

Cancer Research Center of Russian Federation, Moscow, Russia

View larger version (81K): [in a new window]   Fig. 1. Shape and actin cytoskeleton organization of mouse embryo fibroblasts spreading on control substrate. Fluorescent microscopy after staining for actin. Scale bar, 20 µm.

View larger version (13K): [in a new window]   Fig. 2. Dispersion indices of mouse embryo fibroblasts on usual substrate and adhesive strips during spreading (means±s.e.m.).

View larger version (14K): [in a new window]   Fig. 3. Length of mouse embryo fibroblasts on usual substrate and adhesive strips during spreading (means±s.e.m.).

View larger version (12K): [in a new window]   Fig. 4. Proportion of cells with circular bundles of actin filaments during spreading of mouse embryo fibroblasts (means±s.e.m.).

View larger version (50K): [in a new window]   Fig. 5. Morphology and cytoskeleton organization of mouse embryo fibroblasts treated with Taxol on the control substrate or on the substrate with narrow adhesive strips. (a,b) Control cells. (c-f) Taxol-treated cells. (a,c,d) Cells on the control substrate. (b,e,f) Cells on the substrate with narrow adhesive strips. Fluorescent microscopy after staining for actin (a,b,c,e) and tubulin (d,f). Scale bar, 20 µm.

View larger version (15K): [in a new window]   Fig. 6. Length of mouse embryo fibroblasts on control substrate and adhesive strips after Taxol treatment (means±s.e.m.). The difference between length on strips and on plane is highly significant for Taxol-treated culture but not for control culture.

View larger version (30K): [in a new window]   Fig. 7. Morphology and actin cytoskeleton organization of MDCK epitheliocytes treated with scatter factor on the control substrate or substrate with narrow adhesive strips. (a,b) Control cells. (c,d) Scatter-factor-treated cells. (a,c) Cells on the control substrate. (b,d) Cells on the substrate with narrow adhesive strips. Fluorescent microscopy after staining for actin. Scale bar, 20 µm.

View larger version (15K): [in a new window]   Fig. 8. Length of MDCK epitheliocytes on control substrate and adhesive strips after scatter-factor treatment (means±s.e.m.). The difference between length on strips and plane is highly significant for control culture but not for scatter-factor-treated culture.

View larger version (58K): [in a new window]   Fig. 9. Morphology and actin cytoskeleton organization of IAR2 epitheliocytes treated with Y27632 on the control substrate or on the substrate with narrow adhesive strips. (a,c) Control cells. (b,d) Y27632-treated cells. (a,b) Cells on the control substrate. (c,d) Cells on the substrate with narrow adhesive strips. Fluorescent microscopy after staining for actin. Scale bar, 20 µm.

View larger version (14K): [in a new window]   Fig. 10. Length of mouse embryo fibroblasts on control substrate and adhesive strips after Y27632 treatment (means±s.e.m.). Difference between length on strips and plane is highly significant for Y27632-treated culture but not for control culture.

View larger version (13K): [in a new window]   Fig. 11. Length of IAR2 epitheliocytes on control substrate and adhesive strips after Y27632 treatment (means±s.e.m.).