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doi: 10.1242/10.1242/jcs.00633

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Fluorescence in situ hybridization: past, present and future

Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA

View larger version (26K): [in a new window]   Fig. 1. Number of citations in the PubMed database for the keywords 'fluorescence in situ hybridization' by publication year. Widespread application of the technology was not reached until the introduction of easily accessed methods for probe preparation and detection. After this occurred, the technology spread logarithmically.

View larger version (31K): [in a new window]   Fig. 2. State of the art in FISH. (A) Many transcription sites (10) detected using barcoded probes can determine the gene expression pattern of each cell (Levsky et al., 2002) (B-D). Detection of single mRNA molecules using double or triple colored probes (Femino et al., 1998). (E) Detection of 24 chromosomes using spectral imaging (Macville et al., 1997).