QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

This Article Summary Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Foley-Comer, A. J. Articles by Mutsaers, S. E. Search for Related Content PubMed PubMed Citation Articles by Foley-Comer, A. J. Articles by Mutsaers, S. E.

Evidence for incorporation of free-floating mesothelial cells as a mechanism of serosal healing

Department of Medicine, Royal Free and University College Medical School, The Rayne Institute, London, WC1E 6JJ, UK
^* Present address: University Department of Surgery, University of Western Australia, Royal Perth Hospital, Perth, Western Australia, 6000, Australia

View larger version (113K): [in a new window]   Fig. 1. Implantation of fluorescence-labelled cultured mesothelial cells and peritoneal lavage-derived cells on representative areas of serosal injury. DiI-labelled cultured mesothelial cells, characterised by intense, punctate, red fluorescence in cell suspension (inset A), were present on imprints of regenerating mesothelium 5 days post injury (A), whereas DiI-labelled cultured fibroblasts were absent at the same time point (B). DiI-labelled peritoneal lavage cells were also present 8 days post injury (C). DiI-labelled cells (arrowed) were clearly distinguished from adjacent unlabelled cells (arrowheads). PKH26-PCL-labelled phagocytic cells were characterised by intense cytoplasmic red fluorescence in peritoneal lavage fluid obtained 2 days after peritoneal abrasion injury (inset D). Labelled macrophages (arrowed) were implanted onto the wound surface at 3 and 5 days post injury (D and E, respectively) but were absent by 8 days (F). Bars, 10 µm.

View larger version (75K): [in a new window]   Fig. 2. Proliferation of CM-DiI-labelled cultured mesothelial cells in regenerating mesothelium. Immunolocalisation of PCNA 4 days post injury shows proliferating cells in seminiferous tubules (A) and regenerating mesothelium (boxed area and B). The adjacent tissue section shows CM-DiI-labelled mesothelial cells in multiple layers at the wound site (C), some corresponding to PCNA-positive cells (arrowed). Bar, 10 µm.

View larger version (104K): [in a new window]   Fig. 3. Identification of mesothelial cells by HBME-1 immunostaining. HBME-1 expression in normal mesothelium (A). Dual localisation of DiI and HBME-1 on gelatin imprints of regenerating mesothelium, 8 days following injection of DiI-labelled peritoneal lavage cells, demonstrating membranous HBME-1 expression on the surface of implanted DiI-labelled cells (B, arrowed). Bar, 10 µm.

View larger version (142K): [in a new window]   Fig. 4. Incorporation of labelled cells into regenerating mesothelium. ZO-1 expression in normal mesothelium is localised to the plasma membrane (A). Dual localisation of DiI and ZO-1 on imprints of regenerating mesothelium, 5 days following injection of DiI-labelled cultured mesothelial cells (B, arrowed) and 8 days following injection of DiI-labelled peritoneal lavage cells (C, arrowed), demonstrating relocation of ZO-1 to the plasma membrane at points of cell-to-cell contact. Bar, 10 µm.