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Dis1/TOG universal microtubule adaptors - one MAP for all?

¹ The Wellcome Trust Centre for Cell Biology, Institute of Cell and Molecular Biology, The University of Edinburgh, Edinburgh EH9 3JR, UK
² Laboratory of Cell Regulation, Imperial Cancer Research Fund, PO Box 123, 44 Lincoln’s Inn Fields, London WC2A 3PX, UK

View larger version (25K): [in a new window]   Fig. 1. Architecture of the Dis1/TOG family of MAPs. TOG domains (light-blue boxes), HEAT-repeating units (small yellow boxes) (Neuwald and Hirano, 2000), and coiled-coil regions (small grey boxes) are shown in different members of the Dis1/TOG family from various organisms. The last TOG domain in the first and second groups is very degenerate at the primary sequences (Cassimeris et al., 2001).

View larger version (101K): [in a new window]   Fig. 2. Universal localisation to spindle poles - centrosomes, acentrosomal poles and SPBs. (A) Drosophila Msps localises to centrosomes of mitotic metaphase spindles in Drosophila syncytial embryos (red, Msps; green, tubulin; blue, DNA). (B) Msps also localises to acentrosomal spindle poles at female meiosis I (red, Msps; green, tubulin; blue, DNA). (C) Fission yeast Alp14 localises to SPBs (two stronger signals indicated by arrow heads) at mitotic metaphase as well as kinetochores (weaker signals in the centre). Bars, 2 µm.

View larger version (113K): [in a new window]   Fig. 3. Dynamic localisation of fission yeast Alp14 during interphase. Four interphase cells, in which two are in late G2 phase (left), whereas the other two (right) are in post-anaphase and G1 phase, are shown. Live images of Alp14-GFP are shown every 60 seconds, and schematic localisation of Alp14 (green), together with microtubules (red), is depicted in the right-hand panels. The movie of this sequence can be found at http://jcs.biologists.org/supplemental/. Bar, 10 µm.

View larger version (31K): [in a new window]   Fig. 4. Multiple roles of the Dis1/TOG family of MAPs. (A) The Dis1/TOG family of MAPs plays crucial roles in multiple microtubule functions (shown in yellow boxes), which are likely to be mediated by their interaction with various proteins (shown in blue boxes). Microtubules, chromosomes, the Dis1/TOG family of MAPs and the interacting proteins are represented by green, blue, red circles and purple squares, respectively. KC, kinetochore; MT, microtubule. (B) A speculative view of the interaction between Dis1/TOG and their binding partners is illustrated. The C-terminal domain (red oval) is interacting with a microtubule (white and red). The N-terminal TOG domains (blue) are also shown interacting with proteins (MAPs and effector proteins)