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Quarterly Journal of Microscopical Science, Vol s3-99, 231-242, Copyright © 1958 by Company of Biologists

The Histochemistry of Bound Lipids

M. C. BERENBAUM 1

1 Glaxo Laboratories Ltd., Greenford

Many tissue-lipids are bound in varying degrees to other substances, mainly protein, and so are resistant to extraction by organic solvents. Such bound lipids are still present in paraffin sections, but they cannot be satisfactorily demonstrated by conventional use of the Sudans. It has been found that an essential condition for such demonstration is their prior dissociation from their complexes by hydration, heat, or the action of proteolytic enzymes.

Methods are described by which, after routine fixation, bound lipids may be partially dissociated from their complexes and then demonstrated by Sudan black, in paraffin sections or in smears.

These methods show lipids to be present constantly in association with nuclear and cytoplasmic nucleoprotein, in basement membranes, epithelial brush borders, neurones, and many other structures. There is evidence that these lipids do not form a homogeneous class, but vary widely in their physico-chemical natures.






© The Company of Biologists Ltd 1958