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Quarterly Journal of Microscopical Science, Vol s3-99, 67-71, Copyright © 1958 by Company of Biologists

A Simultaneous Coupling Azo Dye Technique Suitable for Whole Mounts

P. R. LEWIS 1

1 Anatomy School, Cambridge

A method based on the familiar coupling azo dye technique has been devised for the study of enzyme distribution in thick specimens. Its application to the study of esterase distribution in whole tadpoles of Xenopus laevis is described in detail. It can also be used to show the distribution of alkaline phosphatase, both in Xenopus tadpoles and in limbs dissected from chick embryos. It has proved useful in embryological studies both for teaching and for research.






© The Company of Biologists Ltd 1958