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Quarterly Journal of Microscopical Science, Vol s3-99, 61-63, Copyright © 1958 by Company of Biologists

A Technique for Marking the Site of Recording with Capillary Micro-electrodes

K. H. BULTITUDE 1

1 Department of Zoology and Comparative Physiology, The University of Birmingham

Records were made from the hind-brain of 15-cm tench (Tinca tinca, Osteichthyes) by the use of capillary micro-electrodes. At the completion of recording the electrodes were left in position and the site of recording was marked by the following method. The Ringer bathing the brain was replaced by a mixture of equal parts of 110 mM FeCl3 and Ringer. After 4 h, ferrocyanide ions were released electrolytically from the micro-electrode. The electrolyte in the micro-electrodes was 2.5 M KCl+0.5 M Na4Fe(CN)8. The optimum quantity of electricity for the electrolysis was found to be 2x10-4 coulombs. The brain was then fixed overnight in 90% alcohol, sectioned at 20 µ, and stained with eosin.

Precipitates of Prussian blue about 20µ in diameter were found at the site of the electrode tip.






© The Company of Biologists Ltd 1958