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Quarterly Journal of Microscopical Science, Vol s3-99, 1-3, Copyright © 1958 by Company of Biologists
1 Department of Zoology, University of Cambridge, and the Marine Station, Millport
Unfertilized eggs of the sea-urchin Echinus esculentus were fixed in potassium permanganate, sectioned, and examined under the electron microscope. A membrane consisting of an outer electron-opaque layer 84 Å wide, an electron-transparent layer 67 Å wide, and an inner electron-opaque layer 66 A wide was observed at the egg surface. No other membrane or electron-opaque layer could be found anywhere within 4µ of the egg surface.