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Quarterly Journal of Microscopical Science, Vol s3-98, 369-375, Copyright © 1957 by Company of Biologists

The Uptake of 35S in Cortical Bone

LORNA LEA 1 and JANET VAUGHAN 1

1 Nuffield Department of Medicine, Oxford

A further study of the uptake of 35S in rabbits and in normal and rachitic puppies has been undertaken. The non-labile 35S present in osteoid tissue is removed by testicular hyaluronidase, but not by staphylococcal hyaluronidase, which suggests that it is chondroitin sulphate A or C. The non-labile 35S is first taken up by non-calcified osteoid tissue in the area giving a pale pink PAS reaction. It is only later found in calcified bone when the tissue ceases to be PAS-positive. Rigid attention to technical etail is essential if comparable results are to be obtained in studies of bone uptake of 35S.






© The Company of Biologists Ltd 1957