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Quarterly Journal of Microscopical Science, Vol s3-97, 155-159, Copyright © 1956 by Company of Biologists

The Histochemical Localization of {beta}-Glucuronidase in the Liver of the Newt

F. BILLETT 1 and S. M. McGEE-RUSSELL 2

1 Department of Zoology and Comparative Anatomy, Oxford, Present addresses; F. Billett, Institute of Animal Genetics, west Mains Road, Edinburgh 9
2 Department of Zoology and Comparative Anatomy, Oxford, Present addresses; S. M. McGee-Russell, Zoology Dept., Birkbeck College, Malet St., London, W.C.I

The localization of {beta}-glucuronidase in the newt liver, by the use of quinolyl-8-glucuronide as a substrate, is described. The technique previously used for the digestive gland of the Roman snail may be applied successfully to the newt tissue. Results suggest, however, that the treatments involved produce considerable destruction of the fine structure of the cytoplasm, and that present techniques based upon the precipitation of the ferric salt of 8-hydroxyquinoline cannot give a fine intracellular localization of the enzyme.






© The Company of Biologists Ltd 1956