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Quarterly Journal of Microscopical Science, Vol s3-97, 17-28, Copyright © 1956 by Company of Biologists

The Site of Damage in Amoebae Exposed to Lethal Concentrations of Methyl Di-({beta}-Chloroethyl)-Amine (a ‘Nitrogen Mustard’)

M. J. ORD 1 and J. F. DANIELLI 1

1 Department of Zoology, King's College, Strand, London, W.C. 2

The technique of nuclear transfer in Amoeba proteus was used to study the extent to which methyl di-({beta}-chloroethyl)-amine damages nucleua and cytoplasm. Results obtained showed that cytoplasm was damaged directly by the drug; exposure of normal cytoplasm to nuclei damaged by {beta}-chloroethylamine for up to 3 days caused no lethal damage. Nuclei were probably directly damaged, but could also be damaged by injured cytoplasm, normal nuclei being lethally damaged when exposed to injured cytoplasm for only a hours. Death from lethal cytoplasmic damage generally occurred within a few days; animals with lethally damaged cytoplasm seldom divided. Death from lethal nuclear damage was delayed in some cases for as long as 6 weeks, such nuclei being able to divide once or twice before death.






© The Company of Biologists Ltd 1956