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Quarterly Journal of Microscopical Science, Vol s3-96, 317-322, Copyright © 1955 by Company of Biologists

Experiments on the Mechanism of Silver Staining

IV. Electron Microscope Studies

A. PETERS 1

1 Department of Zoology, The University of Bristol

Silver-stained sections of nervous tissue were examined with an electron microscope. It has been shown that the developed silver is particulate. These particles are between 30-700 Å in diameter and in nerve-fibres they are disposed along the surface and inside the components of the fibre. When impregnated sections were developed in a glycine physical developer, the mean size of the developed silver particles increased with an increase in the time of development.

In silver-stained sections of human sympathetic ganglion, the silver particles were distributed throughout the cell, but were more densely aggregated in the cell membrane and the nucleus. Erythrocytes show a dense aggregation of silver particles throughout the cytoplasm.






© The Company of Biologists Ltd 1955