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Quarterly Journal of Microscopical Science, Vol s3-96, 35-48, Copyright © 1955 by Company of Biologists

The Histochemical Localization of {beta}-Glucuronidase in the Digestive Gland of the Roman Snail (Helix pomatia)

F. BILLETT 1 and S. M. McGEE-RUSSELL 1

1 Department of Zoology and Comparative Anatomy, University Museum, Oxford

A modification of the histochemical technique for the localization of {beta}-glucuronidase originally suggested by Friedenwald and Becker (1948) has been applied to the digestive gland of the gastropod Helix pomatia. In the original technique the ferric 8-hydroxyquinoline formed by the enzymic hydrolysis of quinolyl-8-glucuronide, in a saturated solutionof ferric 8-hydroxyquinoline, was converted to Prussian blue. The Prussian blue conversion is omitted in the technique described in this paper as it appears to introduce errors in localization. The ferric 8-hydroxyquinoline crystals are sufficiently characteristic to be used as the end-point of the technique. The results obtained suggest that {beta}-glucurcnidase is confined to the digestive cells in the digestive gland of the snail, and is associated with secretory granules in them.






© The Company of Biologists Ltd 1955