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Quarterly Journal of Microscopical Science, Vol s3-94, 303-313, Copyright © 1953 by Company of Biologists

Observations on Substances that react weakly to the Periodic Acid/Schiff Test

ARTHUR J. HALE 1

1 Histology Division, Institute of Physiology, The University, Glasgow

It has been shown that certain mucinous substances which are weakly positive with the periodic acid/Schiff technique stain more strongly after treating them with a solution of sodium hydroxide.

The deepening of the staining reaction is produced by making available, for aldehyde production by periodic acid, more .CHOH.CHOH., .CHOH.CHNH2., or .CHOH .CHNHR. groups. This is done without affecting those sulphate or metaphosphate groups responsible for metachromasia.






© The Company of Biologists Ltd 1953