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Quarterly Journal of Microscopical Science, Vol s3-94, 243-246, Copyright © 1953 by Company of Biologists

Standardization of Methyl Green for Specific Staining of Egg-shell Material in a Trematode

J. D. SMYTH 1

1 Department of Zoology, Trinity College, Dublin

1. The specificity of a number of samples of methyl green for egg-shell material in Fasciola hepatica was investigated.

2. A method for distinguishing between specific and non-specific stains by paper chromatography is described.

3. Specific stains gave almost identical chromatograms with an Rf value of 0.83 for both green and violet constituents. Non-specific samples gave chromatograms with an Rf of 0.65 for the green constituent and an Rf of 0.84 for the violet constituent. The liquid phase used was a mixture of organic solvents in the following proportions: ethanol, 100; water, 80; n-butanol, 100; cellosolve, 100.

4. The application of this technique has led to the production of a methyl green, named Methyl Green M.2, specific for shell material in Fasciola.






© The Company of Biologists Ltd 1953