|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Quarterly Journal of Microscopical Science, Vol s3-93, 119-132, Copyright © 1952 by Company of Biologists
1 Departments of Human Anatomy, and Zoology and Comparative Anatomy, Oxford
1. The optimum conditions for vaccinia virus multiplication in shavings of adult rabbit skin maintained in rocker flasks in a fluid medium have been investigated. Satisfactory virus multiplication occurred in 5 ml. of medium containing 40 per cent, rabbit serum, 40 per cent, phosphate-buffered saline, and 20 per cent, mouse embryo extract; an air atmosphere was used.
2. A growth curve for vaccinia virus grown in vitro under these conditions was obtained by estimating the virus content of cultured skin at known intervals after inoculation. The curve was essentially similar to that obtained by growth of vaccinia in vivo, and could be divided into three phases: (a) during the first few hours infectivity fell, (b) from 10 to 40 hours there was a rapid increase of infectivity, and (c) flattening of the curve started after 40 hours, although some increase of virus continued, until, at 80 hours, the concentration of virus present was at a maximum.
3. An attempt is made to correlate the multiplication of virus with the cytological changes known to occur in infected cells.
