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Quarterly Journal of Microscopical Science, Vol s3-92, 343-346, Copyright © 1951 by Company of Biologists

Intranuclear Birefringent Inclusions, an Artifact Occurring in Paraffin Sections

G. A. NEDZEL M.D.1

1 Laboratory of Pathology and Pharmacology, National Institute of Arthritis and Metabolic Diseases, National Institutes of Health, Public Health Service, Federal Security Agency, Bethesda 14, Md., U.S.A.

Intranuclear birefringent inclusions, apparently paraffin, are often observed in paraffin sections which are dehydrated in acetone instead of alcohol after staining. These inclusions are not removed by prolonged immersion of the sections in xylene or other fat solvents at room temperature. They may be removed from unmounted sections by clearing these sections in xylene after the usual hydration and alcohol dehydration, either before or after staining. They may be removed from resin-mounted sections, but not completely so, by warming the sections in an oven at 58° C. for 15 minutes.






© The Company of Biologists Ltd 1951