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Quarterly Journal of Microscopical Science, Vol s3-91, 348-352, Copyright © 1950 by Company of Biologists

On the Use of Wide Illuminating Cones in the Microscope

A. G. OETTLÉ 1

1 Nuffield Travelling Medical Fellow, Department of Human Anatomy, Oxford

In bright-ground illumination, glare is noticeable when the condenser aperture is equivalent to that of the objective. It can still be detected, though to a greatly reduced extent, after most causes of glare have been avoided. It is suggested that this residual glare is inevitable, and represents the loss of contrast resulting from a relative excess of direct rays, which occurs when the direct light falls in the marginal zone of the objective, since half of the diffracted rays then falls outside the lens.






© The Company of Biologists Ltd 1950