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Quarterly Journal of Microscopical Science, Vol s3-91, 251-277, Copyright © 1950 by Company of Biologists

The Effect of Inhibitory Substances on Cell Division

A Study on Living Cells in Tissue Cultures

A.F. W. HUGHES 1

1 Sir Halley Stewart Research Fellow. From Strangeways Research Laboratory, Cambridge

1. The effect of inhibitory substances upon cell divisions in chick tissue cultures has been, studied mainly by phase-contrast cine-micrography.

2. Fluoride and powerful -SH reactants prevent the entry of cells into prophase within I hour.

3. Aminopterin and colchicine are complete spindle inhibitors; they prevent its formation at the end of prophase, and can act on all metaphase cells in the culture.

4. The effect of fluoride, cyanide, and urethane on the spindle is much less drastic.

5. Most substances can retard nuclear reconstruction in telophase to some extent, but only chloracetophenone acts in the way already described for iodoacetamide.

6. Malonate appears to have no effect on mitosis, and fluoracetate only at very high concentrations.

7. Possible interpretations of some of these results are discussed.






© The Company of Biologists Ltd 1950