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Quarterly Journal of Microscopical Science, Vol s3-91, 73-78, Copyright © 1950 by Company of Biologists

A Study of the Male Germ-cells of the Rat and the Mouse by Phase-contrast Microscopy

R.A. R. GRESSON 1

1 Department of Zoology, University of Edinburgh

1. The cytoplasmic components of the male germ-cells of the rat and the mouse were examined by phase-contrast microscopy. Most of the structures seen in fixed and stained preparations were identified.

2. The localized Golgi material of spermatocytes and spermatids consists of rods and filaments. The mitochondria are granular.

3. The archoplasmic vacuole, proacrosome, developing acrosome, proximal and distal centrioles, and the axial filament were visible. A structure which may be an accessory body was observed in spermatocytes and spermatids. The manchette was visible in late spermatids of the rat but not in those of the mouse.

4. Examination of spermatozoa revealed the acrosome, the post-nuclear cap, the mitochondrial sheath, the ring centriole, and the protoplasmic bead. A granule was sometimes visible in the neck region.






© The Company of Biologists Ltd 1950