Quarterly Journal of Microscopical Science, Vol s3-90, 293-307, Copyright © 1949 by Company of Biologists

Further Remarks on the Golgi Element

¹ Department of Zoology and Comparative Anatomy, Oxford

1. The Golgi element has been reinvestigated in the same kinds of cells as were the subject of the author's 1944 paper.

2. Two new methods have been used, namely, phase-contrast microscopy and an improved form of the sudan black technique, in which the tissues are postchromed at 60° C.

3. The Golgi element consists of separate bodies, spheroid in shape. These Golgi bodies may be simple (i.e. non-vacuolate), or may contain one or more vacuoles. The material of the simple Golgi body and of the externum of the vacuolate body is a lipoid that in some cases can be shown to contain lipine. The secretion-product of the Golgi body originates in the vacuole.

4. The opinion as to the structural plan of the Golgi element set out in the earlier paper has been confirmed in the main. There are, however, two exceptions to this:

(a) The vacuole in the Golgi body does not invariably colour with neutral red, and this dye occasionally causes the appearance of vacuoles not present before, both within the Golgi region and in other parts of the cytoplasm.

(b) ‘Diffuse lipoid’ is not a characteristic feature of the Golgi element.