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Quarterly Journal of Microscopical Science, Vol s3-89, 233-238, Copyright © 1948 by Company of Biologists

A New Method for Oblique Microscopical Illumination

JOHN R. BAKER 1

1 Department of Zoology and Comparative Anatomy, Oxford

The purpose of the method is to facilitate the resolution of extremely fine regularly-repeated markings on microscopical objects. Resolution is obtained by using the objective as an eyepiece to observe the direction and degree of obliquity of the light; these are controlled by movements of the mirror. To enable the objective to be used in this way, the condenser is raised above the position required for so-called ‘critical’ illumination. The diffraction-spectrum formed by the markings on the object is clearly seen while the mirror movements are being made, and it is easy to produce a bright and complete spectrum.






© The Company of Biologists Ltd 1948