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Quarterly Journal of Microscopical Science, Vol s3-88, 115-121, Copyright © 1947 by Company of Biologists

The Histochemical Recognition of Certain Guanidine Derivatives

JOHN R. BAKER 1

1 Department of Zoology and Comparative Anatomy, Oxford

1. A review of known facts indicates that Sakaguchi's test gives a positive reaction with all substances having this formula (and with no others):

where {alpha} is H or CH3 and {beta} is also H or CH3/

2. Arginine, being a constituent of most proteins, is by far the commonest positively-reacting substance that occurs in the tissues of plants and animals. Galegine and agmatine, which also react positively, are of very restricted occurrence.

3. Details are given of a method whereby Sakaguchi's test may be applied to paraffin sections.






© The Company of Biologists Ltd 1947