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Quarterly Journal of Microscopical Science, Vol s3-106, 197-214, Copyright © 1965 by Company of Biologists

Electron Microscope observations on Mitotic Chromosomes

N. A. BARNICOT 1 and H. E. HUXLEY 1

1 Department of Anthropology, University College, London, W.C. 1, and the Medical Research Council Laboratory of Molecular Biology, University Postgraduate Medical School, Hills Road, Cambridge

A method is described by which single mitotic cells growing in tissue culture can be selected under the light microscope and then sectioned for electron-microscopic study. The method has been applied to mitotic cells in newt heart cultures and to monolayer cultures of human fibroblasts. The structure of the chromosomes and of the mitotic apparatus at various phases of mitosis are described and discussed. The effects of pH and of divalent cations on the fixation of chromosomes by buffered osmic mixtures are also considered.

Note:

Part of this work was carried out while H. E. Huxley was working in the Biophysics Department, University College, London, W.C. 1.






© The Company of Biologists Ltd 1965