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Quarterly Journal of Microscopical Science, Vol s3-105, 231-244, Copyright © 1964 by Company of Biologists

Experiments on Embedding Media for Electron Microscopy

S. M. McGEE-RUSSELL 1 and W. C. DE BRUIJN 1

1 Electron Microscopy Laboratory, Virus Research Unit, Medical Research Council Laboratories, Carshalton, Surrey, England, and Centraal Pathologisch Laboratorium, Ziekenhuis Dijkzigt, Rotterdam, Holland

Experiments were conducted to determine the feasibility of improving the trimming and sectioning properties of the polymers glycol methacrylate (Rosenberg, Bartl and Lesko, 1960) and durcupan (X133/1097) (Stäubli, 1960) by combining them with other resins, in particular epon 812. We have also attempted to determine the value of comparative studies of tissue given different fixation and dehydration treatments, prior to embedding in the same final embedding medium.






© The Company of Biologists Ltd 1964