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Quarterly Journal of Microscopical Science, Vol s3-103, 451-456, Copyright © 1962 by Company of Biologists

A Study of the Fixation of the Granular Elements in Rat Pancreas

C. LEVENE 1 and P. FENG 1

1 Departments of Anatomy and Pharmacology, University College of the West Indies, Jamaica

Rat pancreases were fixed in 6 primary fixatives and 16 fixative mixtures. Paraffin sections were stained for {alpha} and zymogen granules with chromalum/haematoxylin/phloxine, and for {beta} granules with aldehyde-fuchsin.

Fixation of {alpha} and {beta} granules was obtained with all fixatives containing formaldehyde or potassium dichromate. Zymogen granules were only fixed by fixatives containing formaldehyde, and then only if acetic acid was omitted.

Formaldehyde alone was adequate for fixation of {alpha}, {beta}, and zymogen granules. For fixation of {alpha} and {beta} granules only, the mixture of formaldehyde and acetic acid was preferable because the sections were technically better.






© The Company of Biologists Ltd 1962