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Quarterly Journal of Microscopical Science, Vol s3-103, 5-15, Copyright © 1962 by Company of Biologists

Experiments on Fixation for Electron Microscopy

1. Unbuffered Osmium Tetroxide

S. K. MALHOTRA 1

1 The Cytological Laboratory, Department of Zoology, University Museum, Oxford

The effect of fixation with a simple solution of osmium tetroxide in distilled water was studied by electron microscopy. Exocrine cells of the pancreas and cells of the proximal tubule of the kidney of the mouse were used as test-objects. Partially prepolymerized methacrylate was used for embedding. There did not appear to be any marked disorganization of the cell inclusions. The appearance of the inclusions in the electron micrographs was similar to what is generally seen after fixation with the buffered osmium tetroxide solution of Palade.






© The Company of Biologists Ltd 1962