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Quarterly Journal of Microscopical Science, Vol s3-101, 465-473, Copyright © 1960 by Company of Biologists

On the Interpretation of Phase-Contrast Images

W. H. STEEL 1 and Y. T. TCHAN 1

1 C.S.I.R.O. Division of Physics, National Standards Laboratory, Sydney, Australia, and the Microbiological Laboratories, University of Sydney

It is shown that the simple theory of phase-contrast microscopy may be extended to take some account of the size of the object by the introduction of an ‘overlap factor’ which expresses the fraction of the diffracted light that passes through the phase-changing region of the phase plate. The extended theory can explain observed effects that cannot be explained even qualitatively if overlap is ignored. The overlap factor is given for disk-shaped objects and an annular phase plate.






© The Company of Biologists Ltd 1960