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Quarterly Journal of Microscopical Science, Vol s3-101, 241-250, Copyright © 1960 by Company of Biologists

A Study of Potassium Permanganate ‘Fixation’ for Electron Microscopy

S. BRADBURY 1 and G. A. MEEK 1

1 Department of Human Anatomy, South Parks Road, Oxford

The action of buffered potassium permanganate as a fixative for electron microscopy has been investigated. Electron contrast has been shown to be produced by the deposition of granular material in the tissue. The particles are about 50 Å in diameter. Actual fixation of the tissue is performed by the dehydrating alcohol.

Histochemical studies have shown that RNA and histones are removed, whereas phospholipid-protein complexes are ‘unmasked’. The reaction of the permanganate with unmasked protein gives rise to high membrane contrast in electron micrographs.






© The Company of Biologists Ltd 1960