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Quarterly Journal of Microscopical Science, Vol s3-101, 39-41, Copyright © 1960 by Company of Biologists

A Modification of the ‘Hermann PO’ Method for Mitochondria

BARBARA M. JORDAN-LUKE 1

1 Cytological Laboratory, Department of Zoology, University Museum, Oxford

Tissues are fixed in Hermann's fluid, postosmicated at 340° C, and embedded in n-butyl methacrylate. Sections are cut with an oblique knife on a sliding microtome, attached to glass slides with albumen, and mounted in DPX or Canada balsam.

Mitochondria are shown in black against a colourless, transparent cytoplasm. They retain their original form. Preparations made in this way are superior to those made by the original Hermann PO technique in the remarkable clarity of the mitochondria and the absence of distortion in the tissue as a whole.

It is confirmed that the presence of acetic acid at 5% in the fixative in no way damages the mitochondria.






© The Company of Biologists Ltd 1960