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Quarterly Journal of Microscopical Science, Vol s3-100, 269-274, Copyright © 1959 by Company of Biologists

The Action of Fixatives on the Unmasking of Lipid

BLANCHE-P. CLAYTON 1

1 Cytological Laboratory, Department of Zoology, University Museum, Oxford

1. The capacity of various primary (unmixed) fixatives to split lipids from lipo-protein complexes was investigated.

2. The lipoproteins that formed the test-objects of the research were those contained in (a) the acroblast of the cricket (Acheta domesticus), and (b) the endoplasmic reticulum (‘ergastoplasm’) of the acinar cell of the pancreas of the laboratory mouse. The two test-objects gave essentially the same results.

3. Of the 16 compounds tried, cadmium chloride is the most powerful unmasker of lipids. Mercuric chloride is also a powerful unmasker, and gives much better fixation. Chloroplatinic acid and formaldehyde are moderately powerful unmaskers.






© The Company of Biologists Ltd 1959