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Quarterly Journal of Microscopical Science, Vol s3-100, 231-239, Copyright © 1959 by Company of Biologists

A Study of Rawitz's ‘Inversion Staining’

ALEKSANDRA PRZELECKA 1

1 Cytological Laboratory, Department of Zoology, University Museum, Oxford, and the Nencki Institute, 3 Pasteur St., Warsaw 22; Nencki Institute

The Rawitz method involves mordanting with tannic acid and potassium antimony tartrate, and staining with basic fuchsine. The mordanting causes basic fuchsine to act as though it were an acid dye (‘inversion staining’).

A modification of the method is described in the present paper. This modification makes it possible to obtain the same results in a shorter time.

The chief substances stained by Rawitz's method are phospholipids, certain proteins, and certain polysaccharides.

Although the method cannot be regarded as a cytochemical test in the strict sense, yet it gives useful indications of chemical composition and in addition is valuable to the morphological cytologist as a technique for showing certain cytoplasmic inclusions (mitotic spindle, acrosome, mitochondria, ‘Golgi apparatus’ of certain cells).






© The Company of Biologists Ltd 1959