PI-3-kinase-dependent membrane recruitment of centaurin-₂ is essential for its effect on ARF6-mediated actin cytoskeleton reorganisation

GTPase activating proteins (GAPs) of the centaurin family regulate the actin cytoskeleton and vesicle trafficking through inactivation of the ADP-ribosylation factor (ARF) family of small GTP-binding proteins. We report the functional characterisation of centaurin-₂, which is structurally related to the centaurin-₁ ARF6 GAP. Centaurin-₂ contains an N-terminal GAP domain followed by two pleckstrin homology (PH) domains (N-PH and C-PH). In vitro, GFP-centaurin-₂ specifically binds the phosphatidylinositol (PI) 3-kinase lipid products, PI 3,4-P₂ and PI 3,4,5-P₃ (PIP₃), through its C-terminal PH domain. In agreement with this observation, GFP-centaurin-₂ was recruited to the plasma membrane from the cytosol in EGF-stimulated cells in a PI-3-kinase-dependent manner. Moreover, the C-PH domain is sufficient and necessary for membrane recruitment of centaurin-₂. Centaurin-₂ shows sustained kinetics of PI-3-kinase-mediated membrane recruitment in EGF-stimulated cells, owing to its binding to PI 3,4-P₂. Centaurin-₂ prevents ARF6 translocation to, and cortical actin formation at, the plasma membrane, which are phenotypic indications for ARF6 activation in EGF-stimulated cells. Moreover, the constitutively active mutant of ARF6 reverses the effect of centaurin-₂ on cortical actin formation. The membrane targeted centaurin-₂ is constitutively active. Together, these studies indicate that centaurin-₂ is recruited in a sustained manner to the plasma membrane through binding to PI 3,4-P₂ and thereby regulates actin reorganisation via ARF6.