spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 9 Aug 2005
doi: 10.1242/jcs.02516

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.02516v1
118/17/3917    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Acquaviva, F.
Right arrow Articles by Cocozza, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Acquaviva, F.
Right arrow Articles by Cocozza, S.

Research Article

Extra-mitochondrial localisation of frataxin and its association with IscU1 during enterocyte-like differentiation of the human colon adenocarcinoma cell line Caco-2


Fabio Acquaviva, Irene De Biase, Luigi Nezi, Giuseppina Ruggiero, Fabiana Tatangelo, Carmela Pisano, Antonella Monticelli, Corrado Garbi, Angela Maria Acquaviva*, and Sergio Cocozza
* Author for correspondence (e-mail: angacqua{at}unina.it)

Friedreich's ataxia is a recessive neurodegenerative disease due to insufficient expression of the mitochondrial protein frataxin. Although it has been shown that frataxin is involved in the control of intracellular iron metabolism, by interfering with the mitochondrial biosynthesis of proteins with iron/sulphur (Fe/S) clusters its role has not been well established. We studied frataxin protein and mRNA expression and localisation during cellular differentiation. We used the human colon adenocarcinoma cell line Caco-2, as it is considered a good model for intestinal epithelial differentiation and the study of intestinal iron metabolism. Here we report that the protein, but not the mRNA frataxin levels, increase during the enterocyte-like differentiation of Caco-2 cells, as well as in in-vivo-differentiated enterocytes at the upper half of the crypt-villus axis. Furthermore, subcellular fractionation and double immunostaining, followed by confocal analysis, reveal that frataxin localisation changes during Caco-2 cell differentiation. In particular, we found an extramitochondrial localisation of frataxin in differentiated cells. Finally, we demonstrate a physical interaction between extramitochondrial frataxin and IscU1, a cytoplasmic isoform of the human Fe/S cluster assembly machinery. Based on our data, we postulate that frataxin could be involved in the biosynthesis of iron-sulphur proteins not only within the mitochondria, but also in the extramitochondrial compartment. These findings might be of relevance for the understanding of both the pathogenesis of Friedreich's ataxia and the basic mechanism of Fe/S cluster biosynthesis.


This article has been cited by other articles:


Home page
 Hum Mol GenetHome page
S. Schmucker, M. Argentini, N. Carelle-Calmels, A. Martelli, and H. Puccio
The in vivo mitochondrial two-step maturation of human frataxin
Hum. Mol. Genet., November 15, 2008; 17(22): 3521 - 3531.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Long, M. Jirku, F. J. Ayala, and J. Lukes
Mitochondrial localization of human frataxin is necessary but processing is not for rescuing frataxin deficiency in Trypanosoma brucei
PNAS, September 9, 2008; 105(36): 13468 - 13473.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
O. Stehling, D. J. A. Netz, B. Niggemeyer, R. Rosser, R. S. Eisenstein, H. Puccio, A. J. Pierik, and R. Lill
Human Nbp35 Is Essential for both Cytosolic Iron-Sulfur Protein Assembly and Iron Homeostasis
Mol. Cell. Biol., September 1, 2008; 28(17): 5517 - 5528.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
A. Martelli, M. Wattenhofer-Donze, S. Schmucker, S. Bouvet, L. Reutenauer, and H. Puccio
Frataxin is essential for extramitochondrial Fe S cluster proteins in mammalian tissues
Hum. Mol. Genet., November 15, 2007; 16(22): 2651 - 2658.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
Y. Shan, E. Napoli, and G. Cortopassi
Mitochondrial frataxin interacts with ISD11 of the NFS1/ISCU complex and multiple mitochondrial chaperones
Hum. Mol. Genet., April 15, 2007; 16(8): 929 - 941.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. Biederbick, O. Stehling, R. Rosser, B. Niggemeyer, Y. Nakai, H.-P. Elsasser, and R. Lill
Role of Human Mitochondrial Nfs1 in Cytosolic Iron-Sulfur Protein Biogenesis and Iron Regulation
Mol. Cell. Biol., August 1, 2006; 26(15): 5675 - 5687.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
I. Condo, N. Ventura, F. Malisan, B. Tomassini, and R. Testi
A Pool of Extramitochondrial Frataxin That Promotes Cell Survival
J. Biol. Chem., June 16, 2006; 281(24): 16750 - 16756.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2005