The fully linked HTML version of this article has now been published.
JCS ePress
online publication date 26 Apr 2005
doi: 10.1242/jcs.02355
Research Article
Functional contribution of Pds5 to cohesin-mediated cohesion in human cells and Xenopus egg extracts
Ana Losada,
Tomoki Yokochi,
and
Tatsuya Hirano*
* Author for correspondence (e-mail: hirano{at}cshl.edu)
Sister chromatid cohesion is essential for proper segregation of the genome in mitosis and meiosis. Central to this process is cohesin, a multi-protein complex conserved from yeast to human. Previous genetic studies in fungi have identified Pds5/BimD/Spo76 as an additional factor implicated in cohesion. Here we describe the biochemical and functional characterization of two Pds5-like proteins, Pds5A and Pds5B, from vertebrate cells. In HeLa cells, Pds5 proteins physically interact with cohesin and associate with chromatin in a cohesin-dependent manner. Depletion of the cohesin subunit Scc1 by RNA interference leads to the assembly of chromosomes with severe cohesion defects. A similar yet milder set of defects is observed in cells with reduced levels of Pds5A or Pds5B. In Xenopus egg extracts, mitotic chromosomes assembled in the absence of Pds5A and Pds5B display no discernible defects in arm cohesion, but centromeric cohesion is apparently loosened. Unexpectedly, these chromosomes retain an unusually high level of cohesin. Thus, Pds5 proteins seem to affect the stable maintenance of cohesin-mediated cohesion and its efficient dissolution during mitosis. We propose that Pds5 proteins play both positive and negative roles in sister chromatid cohesion, possibly by directly modulating the dynamic interaction of cohesin with chromatin. This idea would explain why cells lacking Pds5 function display rather complex and diverse phenotypes in different organisms.
This article has been cited by other articles:
|
|
|
|
 
J.-M. Peters, A. Tedeschi, and J. Schmitz
The cohesin complex and its roles in chromosome biology
Genes & Dev.,
November 15, 2008;
22(22):
3089 - 3114.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. A. Diaz-Martinez, J. F. Gimenez-Abian, and D. J. Clarke
Chromosome cohesion - rings, knots, orcs and fellowship
J. Cell Sci.,
July 1, 2008;
121(13):
2107 - 2114.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Storlazzi, S. Tesse, G. Ruprich-Robert, S. Gargano, S. Poggeler, N. Kleckner, and D. Zickler
Coupling meiotic chromosome axis integrity to recombination
Genes & Dev.,
March 15, 2008;
22(6):
796 - 809.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. D. Barber, K. McManus, K. W. Y. Yuen, M. Reis, G. Parmigiani, D. Shen, I. Barrett, Y. Nouhi, F. Spencer, S. Markowitz, et al.
Chromatid cohesion defects may underlie chromosome instability in human colorectal cancers
PNAS,
March 4, 2008;
105(9):
3443 - 3448.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Zhang, S. Jain, H. Song, M. Fu, R. O. Heuckeroth, J. M. Erlich, P. Y. Jay, and J. Milbrandt
Mice lacking sister chromatid cohesion protein PDS5B exhibit developmental abnormalities reminiscent of Cornelia de Lange syndrome
Development,
September 1, 2007;
134(17):
3191 - 3201.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. V. Skibbens, M. Maradeo, and L. Eastman
Fork it over: the cohesion establishment factor Ctf7p and DNA replication
J. Cell Sci.,
August 1, 2007;
120(15):
2471 - 2477.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Barbosa, N. Kimm, and R. Lehmann
A Maternal Screen for Genes Regulating Drosophila Oocyte Polarity Uncovers New Steps in Meiotic Progression
Genetics,
August 1, 2007;
176(4):
1967 - 1977.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Guacci
Sister chromatid cohesion: the cohesin cleavage model does not ring true
Genes Cells,
June 1, 2007;
12(6):
693 - 708.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D.-Q. Ding, N. Sakurai, Y. Katou, T. Itoh, K. Shirahige, T. Haraguchi, and Y. Hiraoka
Meiotic cohesins modulate chromosome compaction during meiotic prophase in fission yeast
J. Cell Biol.,
August 14, 2006;
174(4):
499 - 508.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Toyoda and M. Yanagida
Coordinated Requirements of Human Topo II and Cohesin for Metaphase Centromere Alignment under Mad2-dependent Spindle Checkpoint Surveillance
Mol. Biol. Cell,
May 1, 2006;
17(5):
2287 - 2302.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Dorsett, J. C. Eissenberg, Z. Misulovin, A. Martens, B. Redding, and K. McKim
Effects of sister chromatid cohesion proteins on cut gene expression during wing development in Drosophila
Development,
November 1, 2005;
132(21):
4743 - 4753.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Losada and T. Hirano
Dynamic molecular linkers of the genome: the first decade of SMC proteins
Genes & Dev.,
June 1, 2005;
19(11):
1269 - 1287.
[Abstract]
[Full Text]
[PDF]
|
|
|
© The Company of Biologists Ltd 2005
