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JCS ePress online publication date 15 Feb 2005
doi: 10.1242/jcs.01634

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Research Article

Cadherin switching: essential for behavioral but not morphological changes during an epithelium-to-mesenchyme transition


Masato Maeda, Keith R. Johnson, and Margaret J. Wheelock*
* Author for correspondence (e-mail: mwheelock{at}unmc.edu)

Epithelium-to-mesenchyme transitions (EMTs) are characterized by morphological and behavioral changes in cells. During an EMT, E-cadherin is downregulated while N-cadherin is upregulated. The goal of this study was to understand the role cadherin switching plays in EMT using a classical model system: transforming growth factor {beta}1 (TGF-{beta}1)-mediated EMT in mammary epithelial cells. We showed that stress fibers and focal adhesions are increased, and cell-cell junctions are decreased in response to TGF-{beta}1. Moreover, these changes were reversible upon removal of TGF-{beta}1. Downregulation of E-cadherin and upregulation of N-cadherin were both transcriptional. Neither experimental knockdown nor experimental overexpression of N-cadherin interfered with the morphological changes. In addition, the morphological changes associated with EMT preceded the downregulation of E-cadherin. Interestingly, TGF-{beta}1-induced motility in N-cadherin-knockdown cells was significantly reduced. Together, these data suggest that cadherin switching is necessary for increased motility but is not required for the morphological changes that accompany EMT.


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