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Journal of Cell Science, Vol 99, 57-66, Copyright © 1991 by Company of Biologists
Submitted on January 2, 1991
Accepted on February 20, 1991
1 Department of Ultrastructural Research, The Tokyo Metropolitan Institute of Medical Science, Tokyo 113, Japan
2 Department of Molecular Biology, Faculty of Science, Nagoya University, Nagoya 464, Japan
3 Department of Ultrastructural Research, The Tokyo Metropolitan Institute of Medical Science, Tokyo 113, Japan; National Institute for Physiological Sciecnes, Okazaki 444, Japan
Author for correspondence: Aichi Prefectural University of Fine Art, Nagakute-cho, Aichi 480-11, Japan
The in situ organization of the inner dynein arms of Chlamydomonas axonemes was investigated by analyzing a number of tilt-series thin-section electron micrographs taken with improved fixation and staining protocols. The use of mutant axonemes lacking the outer dynein arms and the radial spokes enabled us to detect a novel feature: inner-arm projections arranged in two rows. The arrangement of projections within each row was deduced by making a model that fitted both tilt-series longitudinal thin-section images and cross-section images. The model suggested that, within a repeating unit of 96 nm, one of the two rows, positioned more centrifugally, consists of four discrete domains and the other, positioned more centripetally, consists of three pairs of globular structures. Whereas the centripetal row resembles the inner-arm arrangement reported previously, the centrifugal row appears to have been identified for the first time in this study. These findings make necessary the re-examination of the arrangement of various inner-arm subspecies within the axoneme.
Key words: dyein arm, Chlamydomonas, mutant, flagella, tilted images
Submitted on January 2, 1991
Accepted on February 20, 1991
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