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Journal of Cell Science, Vol 98, Issue 1 85-89, Copyright © 1991 by Company of Biologists
JOURNAL ARTICLES |
L Coleman and A Harris
Division of Medical and Molecular Genetics, UMDS Guy's Campus, London Bridge, London, UK.
The epithelia lining the vas deferens and epididymis are directly involved in the pathology of the autosomal recessive disease cystic fibrosis (CF). We have established culture systems for these epithelial cells. Long-term cell lines have now been generated from these primary epithelial cells by transformation with a plasmid containing an origin-defective simian virus 40 (SV40). Lines have been established from vas deferens and epididymis and both maintain expression of the CF gene.
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M. Bertog, D. J. Smith, A. Bielfeld-Ackermann, J. Bassett, D. J. P. Ferguson, C. Korbmacher, and A. Harris Ovine male genital duct epithelial cells differentiate in vitro and express functional CFTR and ENaC Am J Physiol Cell Physiol, May 1, 2000; 278(5): C885 - C894. [Abstract] [Full Text] [PDF]
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 A. N. Smith, M. L. Barth, T. L. McDowell, D. S. Moulin, H. N. Nuthall, M. A. Hollingsworth, and A. Harris A Regulatory Element in Intron 1 of the Cystic Fibrosis Transmembrane Conductance Regulator Gene J. Biol. Chem., April 26, 1996; 271(17): 9947 - 9954. [Abstract] [Full Text] [PDF]
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J. Chambers and A Harris Expression of the cystic fibrosis gene and the major pancreatic mucin gene, MUC1, in human ductal epithelial cells J. Cell Sci., January 6, 1993; 105(2): 417 - 422. [Abstract] [PDF]
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