|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 98, Issue 1 37-43, Copyright © 1991 by Company of Biologists
JOURNAL ARTICLES |
G Joswig, C Petzelt and D Werner
Institute of Cell and Tumor Biology, German Cancer Research Center, Heidelberg, FRG.
Screening of an induced Ehrlich ascites cell-derived lambda gt11 cDNA library with an antibody (GP1), immunoreacting specifically with centrosomal antigen(s) of interphase and mitotic cells of different species, released a partial cDNA clone (lambda P10A) encoding the carboxy-terminal section of a centrosome-specific antigen. This specificity of the clone lambda P10A could be verified by lacZ-directed antigen expression from Escherichia coli Y1089 lysogenized with the recombinant phage lambda P10A and subsequent production of centrosome-specific antibodies by means of the recombinant antigen. Using the lambda P10A insert as a probe, two types of cDNA clones were identified in a lambda gt10 cDNA library by plaque-hybridization. The inserts of PN1 type clones were 1.2 kb (kilobases) and those of PN5 type clones were 2.2 kb in length. The DNA sequence of a PN1 type clone revealed its full-length cDNA nature. The open reading frame of PN1 encodes a rather hydrophilic and highly charged 34.5 x 10(3) Mr polypeptide comprising short but apparently significant strings of 100% sequence identity with the major nuclear lamina polypeptides lamins A/C and lamin B. Restriction enzyme mapping of PN1 and PN5 inserts, cross-hybridization experiments and comparison of overlapping DNA sequences indicate that the 1.2 kb and 2.2 kb cDNAs code for the same 34.5 x 10(3) Mr polypeptide, termed centrosomin A. Western blots of Ehrlich ascites cell proteins show a second, larger GP1 antigen (centrosomin B) whose cDNA has not been cloned. It remains to be investigated whether centrosomin B is encoded by a second mRNA or whether it reflects an oligomeric or a postranslationally modified form of centrosomin A.
This article has been cited by other articles:
|
|
 |
|
  K. R. Johnson, W. C. Merrick, W. L. Zoll, and Y. Zhu Identification of cDNA Clones for the Large Subunit of Eukaryotic Translation Initiation Factor 3. COMPARISON OF HOMOLOGUES FROM HUMAN, NICOTIANA TABACUM, CAENORHABDITIS ELEGANS, AND SACCHAROMYCES CEREVISIAE J. Biol. Chem., March 14, 1997; 272(11): 7106 - 7113. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C Petzelt, G Joswig, A Mincheva, P Lichter, H Stammer, and D Werner The centrosomal protein centrosomin A and the nuclear protein centrosomin B derive from one gene by post-transcriptional processes involving RNA editing J. Cell Sci., January 10, 1997; 110(20): 2573 - 2578. [Abstract] [PDF]
|
|
|
|
|
|
A Vassilev, M Kimble, C. Silflow, M LaVoie, and R Kuriyama Identification of intrinsic dimer and overexpressed monomeric forms of gamma-tubulin in Sf9 cells infected with baculovirus containing the Chlamydomonas gamma-tubulin sequence J. Cell Sci., January 3, 1995; 108(3): 1083 - 1092. [Abstract] [PDF]
|
|
|
|
|
|
W Steffen, E. Fajer, and R. Linck Centrosomal components immunologically related to tektins from ciliary and flagellar microtubules J. Cell Sci., January 8, 1994; 107(8): 2095 - 2105. [Abstract] [PDF]
|
|
|
|
|
|
K Rothbarth, C Petzelt, X Lu, I. Todorov, G Joswig, R Pepperkok, W Ansorge, and D Werner cDNA-derived molecular characteristics and antibodies to a new centrosome-associated and G2/M phase-prevalent protein J. Cell Sci., January 1, 1993; 104(1): 19 - 30. [Abstract] [PDF]
|
|
|
|
|
|
I. Verde, G. Pahlke, M. Salanova, G. Zhang, S. Wang, D. Coletti, J. Onuffer, S.-L. C. Jin, and M. Conti Myomegalin Is a Novel Protein of the Golgi/Centrosome That Interacts with a Cyclic Nucleotide Phosphodiesterase J. Biol. Chem., March 30, 2001; 276(14): 11189 - 11198. [Abstract] [Full Text] [PDF]
|
|
