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Journal of Cell Science, Vol 97, 553-563, Copyright © 1990 by Company of Biologists

Submitted on June 11, 1990
Accepted on August 15, 1990

Identification of proteins associated with microtubule-organizing centres and filaments in the oral apparatus of the ciliate Paramecium tetraurelia

GUY KERYER 1, FRANCINE IFTODE 2, and MICHEL BORNENS 1

1 Centre de Génétique Moléculaire, Centre National de la Recherche Scientifique, 91198, Gif/Yvette, France
2 Laboratoire de Biologie Cellulaire 4, Bat. 444. Universite Paris sud, 91405, Orsay-cedex, France

Author for correspondence

In an effort to study the assembly of microtubules in ciliates we have isolated the oral apparatus of Paramecium tetraurelia, a major microtubuie-organizing centre (MTOC) and identified several proteins localized in the fibrillar system associated with basal bodies. Using a monoclonal antibody raised against human centrosomes (CTR210) and a polyclonal antibody (OF1) directed against a 87x103Mr protein of the oral apparatus of Tetrahymena, we observed the same decoration of the oral apparatus of Paramecium, both in situ and after isolation. Ultrastructural localization further showed the presence of the antigens in the fibrillar network that forms a layer under almost all the buccal cavity in close apposition to the basal bodies. CTR210 recognized two sets of polypeptides of Mr72 and 80x103, whereas OF1 recognized a single polypeptide of Mr87x103. Only the 80x103Mr polypeptide was also decorated with the monoclonal antibody MPM-2, previously shown to decorate the oral apparatus of Paramecium and known to react with phosphorylated epitopes in a large variety of MTOCs. All the proteins identified with the three antibodies are insoluble at high ionic strength, display several isoforms and apparently belong to the same fibrillar material. The function of this material in the spatial organization, the structure, and activity of the MTOCs is discussed.

Key words: microtubule-organizing centres, oral filaments, Paramecium gullet

Submitted on June 11, 1990
Accepted on August 15, 1990




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© The Company of Biologists Ltd 1990