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Journal of Cell Science, Vol 95, Issue 1 49-57, Copyright © 1990 by Company of Biologists
JOURNAL ARTICLES |
R Woodward and K Gull
Biological Laboratory, The University, Canterbury, Kent, UK.
We have used immunofluorescent detection of 5-bromo-2-deoxyuridine-substituted DNA in order to determine the timing of initiation and the duration of nuclear and kinetoplast S-phases within the procyclic stage of the Trypanosoma brucei cell cycle. Both nuclear and kinetoplast S-phases were shown to be periodic, occupying 0.18 and 0.12 of the unit cell cycle, respectively. In addition, initiation of both of these S-phases were in approximate synchrony, differing by only 0.03 of the unit cell cycle. We have also used a monoclonal antibody that recognises the basal bodies of T. brucei in order to visualise cells possessing a new pro-basal body and hence determine the time of pro-basal body formation within the cell cycle. Pro-basal body formation occurred within a few minutes of the initiation of nuclear S-phase, at 0.41 of the unit cell cycle. This provides detection of the earliest known cell cycle event in T. brucei at the level of the light microscope. Cell cycle events including initiation of nuclear and kinetoplast DNA replication and pro-basal body formation may be strictly coordinated in T. brucei in order to maintain the precise single-mitochondrion (kinetoplast), singleflagellum status of the interphase cell.
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A Das, M Gale, V Carter, and M Parsons The protein phosphatase inhibitor okadaic acid induces defects in cytokinesis and organellar genome segregation in Trypanosoma brucei J. Cell Sci., January 12, 1994; 107(12): 3477 - 3483. [Abstract] [PDF]
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S. Pasion, G. Brown, L. Brown, and D. Ray Periodic expression of nuclear and mitochondrial DNA replication genes during the trypanosomatid cell cycle J. Cell Sci., January 12, 1994; 107(12): 3515 - 3520. [Abstract] [PDF]
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C. Vedrenne, C. Giroud, D. R. Robinson, S. Besteiro, C. Bosc, F. Bringaud, and T. Baltz Two Related Subpellicular Cytoskeleton-associated Proteins in Trypanosoma brucei Stabilize Microtubules Mol. Biol. Cell, March 1, 2002; 13(3): 1058 - 1070. [Abstract] [Full Text] [PDF]
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