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Journal of Cell Science, Vol 94, 51-59, Copyright © 1989 by Company of Biologists

Submitted on February 14, 1989
Accepted on May 31, 1989

An experimental approach to nucleolar organization in plant cells: a morphological, cytochemical and quantitative study

FRANCISCO JAVIER MORENO 1, ROSA MARIA RODRIGO 1, and GREGORIO GARCIA-HERDUGO 1

1 Departamento de Biologfa Celular, Facultad de Biologfa, Universidad de Sevilla, 41012-Sevilla, Spain

Author for correspondence

We have used morphological, cytochemical and immunocytochemical techniques, along with transcription inhibitors, to try to understand the nucleolar organization in Allium cepa root meristem cells.

Our results reveal that the fibrillar component shows intense staining after using the NOR-silver technique, and gold particle labelling after incubation with DNase-gold and RNase-gold complexes. These different techniques reveal a reticular structure of the fibrillar component, supported by a central axis that is circular in transverse section. These structures are usually considered to be fibrillar centres, but we have called them clear areas, since we find in their interior neither Ag-NOR proteins nor labelling after incubation with nuclease-gold or anti-DNA.

Taking into account the specific localization of the Ag-NOR proteins, our cytochemical and quantitative results, after applying NOR-silver staining in nucleoli of control cells and cells treated with transcription inhibitors, help us to a better understanding of the mechanisms of action of these drugs. Our results support a structural and functional role for the Ag-NOR proteins throughout the course of transcription.

Key words: Allium cepa, actinomycin D, anti-DNA, cordycepin, NOR-silver staining, nuclease-gold complex, nucleolus

Submitted on February 14, 1989
Accepted on May 31, 1989




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[Abstract] [PDF]


© The Company of Biologists Ltd 1989